![]() The QuickPick™ DNA Fragment kit provides a fast and simple means of isolating DNA fragments from gel or from liquid solutions. The kit and Bio-Nobile's PickO™ gel excisers are suitable for use with standard and low-melting agarose gels in TAE or TBE buffer. The technique does not require organic solvents and eliminates the need for repeated centrifugation, vacuum filtration or column separation. ![]() Purified DNA fragments can be used for downstream applications such as automated fluorescent DNA sequencing, manual DNA sequencing, PCR, in vitro transcription/translation, restriction mapping, restriction enzyme digestion, cloning and labelling. metal affinity kit for proteins, 48 tal affinity kit for proteins, 8 preps. The QuickPick IMAC kits provide a fast and simple means for recombinant histidine-tagged (His-tag) protein purification based on metal affinity using Ni 2+. Immobilized metal ion affinity chromatography (IMAC) was originally introduced as a method for group separation, but today it represents one of the most important tools for the single-step purification of proteins based on polyhistidine tags. The principle of the mechanism is based on the interaction between a metal ion coordinated to a covalently bound chelating ligand and a His-tag protein. The QuickPick™ CM and DEAE kits are based on magnetic cellulose particles coated with carboxymethyl (CM) groups or diethylaminoethyl (DEAE) groups. ![]() Different types of proteins will bind to the coated magnetic particles with affinities that depend on both the conditions used and the types and number of individual charged groups.
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